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recombinant murine wisp1  (R&D Systems)


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    Structured Review

    R&D Systems recombinant murine wisp1
    (A) <t>Wisp1</t> mRNA expression in primary adult mouse cardiac fibroblasts following TGFβ1 (10 ng/mL) treatment for 24–72 h. (B) Representative immunofluorescence images of α-SMA (green), vimentin (red), and DAPI (blue) after 72 h treatment with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (C, D) Representative Western blot (C) and densitometric quantification (D) of α-SMA normalized to GAPDH. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05.
    Recombinant Murine Wisp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+wisp1/Recombinant+Human+TGF-beta+1+(Human+Cell-expressed)+Protein/bio_rxiv__64898__2026__02__17__706476-41-30-35
    Average 96 stars, based on 241 article reviews
    recombinant murine wisp1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state"

    Article Title: WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state

    Journal: bioRxiv

    doi: 10.64898/2026.02.17.706476

    (A) Wisp1 mRNA expression in primary adult mouse cardiac fibroblasts following TGFβ1 (10 ng/mL) treatment for 24–72 h. (B) Representative immunofluorescence images of α-SMA (green), vimentin (red), and DAPI (blue) after 72 h treatment with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (C, D) Representative Western blot (C) and densitometric quantification (D) of α-SMA normalized to GAPDH. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05.
    Figure Legend Snippet: (A) Wisp1 mRNA expression in primary adult mouse cardiac fibroblasts following TGFβ1 (10 ng/mL) treatment for 24–72 h. (B) Representative immunofluorescence images of α-SMA (green), vimentin (red), and DAPI (blue) after 72 h treatment with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (C, D) Representative Western blot (C) and densitometric quantification (D) of α-SMA normalized to GAPDH. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05.

    Techniques Used: Expressing, Immunofluorescence, Western Blot

    Primary adult mouse cardiac fibroblasts were embedded in floating collagen gels and treated for 24 h with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (A) Representative gel images at 0 h and 24 h. (B) Quantification of gel area (% contraction; n = 4 isolations, 2 males & 2 females). (C–D) Confluent fibroblast monolayers were scratched and imaged every 2 hours for 24 hours using the same treatment conditions. (C) Representative bright-field images at time point 0 and 14. (D) Quantification of wound closure (% of gap closed relative to time 0; n = 8 isolations, 4 male & 4 female). Data are mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons; *p ≤ 0.05.
    Figure Legend Snippet: Primary adult mouse cardiac fibroblasts were embedded in floating collagen gels and treated for 24 h with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (A) Representative gel images at 0 h and 24 h. (B) Quantification of gel area (% contraction; n = 4 isolations, 2 males & 2 females). (C–D) Confluent fibroblast monolayers were scratched and imaged every 2 hours for 24 hours using the same treatment conditions. (C) Representative bright-field images at time point 0 and 14. (D) Quantification of wound closure (% of gap closed relative to time 0; n = 8 isolations, 4 male & 4 female). Data are mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons; *p ≤ 0.05.

    Techniques Used:

    Related Articles

    Recombinant:

    Article Title: Matricellular Protein WISP2 Is an Endogenous Inhibitor of Collagen Linearization and Cancer Metastasis
    Article Snippet: To prepare collagen solutions, Col I in 0.01 N HCl [PureCol (atelotype I collagen), Advanced Biomatrix, catalog no. 5005] or telo-Col I [RatCol (telo-type I collagen), Advanced Biomatrix, catalog no. 5153] was neutralized (to pH 7.0 0.2) on ice by adding 0.1 mol/L NaOH andmixed with 10 PBS to yield a stock solution containing 2mg/mL ( 6.67 mmol/L) collagen in 1 PBS. .. Recombinant murine WISP1 (R&D Systems, catalog no. 1680-WS) in PBS or recombinant human WISP2 (PeproTech, catalog no. 120-16) were added at a final concentration of 50 and 100 mg/mL, respectively, unless otherwise indicated. .. The pH of the final collagen þ analyte solutions was measured with an Orion PerpHecT ROSS Combination pH Micro Electrode (Thermo Fisher Scientific) and was confirmed to be constant across conditions.

    Article Title: WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state
    Article Snippet: .. For treatments, CFs were seeded in 12- or 24-well plates in DMEM + 10% FBS and treated for 72 h with recombinant human TGFβ1 (10 ng/mL; R&D Systems, 7754-BH) and/or recombinant murine WISP1 (500 ng/mL; R&D Systems, 1680-WS-050). .. Where indicated, CFs were serum-starved in Opti-MEM (Gibco) and pre-treated with SB203580 (10 μM; Tocris Bioscience) for 30 min before addition of growth factors.

    Article Title: The tumor cell‐secreted matricellular protein WISP 1 drives pro‐metastatic collagen linearization
    Article Snippet: .. Recombinant murine WISP1 (R&D systems [produced in mammalian cells]) or recombinant human WISP1 (PeproTech [produced in E. coli ]) was added at a final concentration of 50 μg/ml (1.26 μM) in PBS unless otherwise indicated. .. Fibrinogen (Sigma) and bovine serum albumin (BSA) were added at final concentrations of 270 μg/ml (794 nM) and 100 μg/ml (1.5 μM) in PBS, respectively.

    Concentration Assay:

    Article Title: Matricellular Protein WISP2 Is an Endogenous Inhibitor of Collagen Linearization and Cancer Metastasis
    Article Snippet: To prepare collagen solutions, Col I in 0.01 N HCl [PureCol (atelotype I collagen), Advanced Biomatrix, catalog no. 5005] or telo-Col I [RatCol (telo-type I collagen), Advanced Biomatrix, catalog no. 5153] was neutralized (to pH 7.0 0.2) on ice by adding 0.1 mol/L NaOH andmixed with 10 PBS to yield a stock solution containing 2mg/mL ( 6.67 mmol/L) collagen in 1 PBS. .. Recombinant murine WISP1 (R&D Systems, catalog no. 1680-WS) in PBS or recombinant human WISP2 (PeproTech, catalog no. 120-16) were added at a final concentration of 50 and 100 mg/mL, respectively, unless otherwise indicated. .. The pH of the final collagen þ analyte solutions was measured with an Orion PerpHecT ROSS Combination pH Micro Electrode (Thermo Fisher Scientific) and was confirmed to be constant across conditions.

    Article Title: The tumor cell‐secreted matricellular protein WISP 1 drives pro‐metastatic collagen linearization
    Article Snippet: .. Recombinant murine WISP1 (R&D systems [produced in mammalian cells]) or recombinant human WISP1 (PeproTech [produced in E. coli ]) was added at a final concentration of 50 μg/ml (1.26 μM) in PBS unless otherwise indicated. .. Fibrinogen (Sigma) and bovine serum albumin (BSA) were added at final concentrations of 270 μg/ml (794 nM) and 100 μg/ml (1.5 μM) in PBS, respectively.

    Produced:

    Article Title: The tumor cell‐secreted matricellular protein WISP 1 drives pro‐metastatic collagen linearization
    Article Snippet: .. Recombinant murine WISP1 (R&D systems [produced in mammalian cells]) or recombinant human WISP1 (PeproTech [produced in E. coli ]) was added at a final concentration of 50 μg/ml (1.26 μM) in PBS unless otherwise indicated. .. Fibrinogen (Sigma) and bovine serum albumin (BSA) were added at final concentrations of 270 μg/ml (794 nM) and 100 μg/ml (1.5 μM) in PBS, respectively.



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    recombinant murine wisp1  (R&D Systems)
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    R&D Systems recombinant murine wisp1
    (A) <t>Wisp1</t> mRNA expression in primary adult mouse cardiac fibroblasts following TGFβ1 (10 ng/mL) treatment for 24–72 h. (B) Representative immunofluorescence images of α-SMA (green), vimentin (red), and DAPI (blue) after 72 h treatment with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (C, D) Representative Western blot (C) and densitometric quantification (D) of α-SMA normalized to GAPDH. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05.
    Recombinant Murine Wisp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+wisp1/Recombinant+Human+TGF-beta+1+(Human+Cell-expressed)+Protein/bio_rxiv__64898__2026__02__17__706476-41-30-35
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    (A) <t>Wisp1</t> mRNA expression in primary adult mouse cardiac fibroblasts following TGFβ1 (10 ng/mL) treatment for 24–72 h. (B) Representative immunofluorescence images of α-SMA (green), vimentin (red), and DAPI (blue) after 72 h treatment with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (C, D) Representative Western blot (C) and densitometric quantification (D) of α-SMA normalized to GAPDH. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05.
    Recombinant Human Murine Wisp1 Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+wisp1/human+recombinant+wisp1+protein/us10272154-524-9-15
    Average 90 stars, based on 1 article reviews
    recombinant human murine wisp1 proteins - by Bioz Stars, 2026-09
    90/100 stars
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    Image Search Results


    (A) Wisp1 mRNA expression in primary adult mouse cardiac fibroblasts following TGFβ1 (10 ng/mL) treatment for 24–72 h. (B) Representative immunofluorescence images of α-SMA (green), vimentin (red), and DAPI (blue) after 72 h treatment with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (C, D) Representative Western blot (C) and densitometric quantification (D) of α-SMA normalized to GAPDH. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05.

    Journal: bioRxiv

    Article Title: WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state

    doi: 10.64898/2026.02.17.706476

    Figure Lengend Snippet: (A) Wisp1 mRNA expression in primary adult mouse cardiac fibroblasts following TGFβ1 (10 ng/mL) treatment for 24–72 h. (B) Representative immunofluorescence images of α-SMA (green), vimentin (red), and DAPI (blue) after 72 h treatment with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (C, D) Representative Western blot (C) and densitometric quantification (D) of α-SMA normalized to GAPDH. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05. Data are mean ± SEM from ≥3 independent isolations. Statistical analysis by one-way ANOVA with Tukey’s post hoc test; *p < 0.05.

    Article Snippet: For treatments, CFs were seeded in 12- or 24-well plates in DMEM + 10% FBS and treated for 72 h with recombinant human TGFβ1 (10 ng/mL; R&D Systems, 7754-BH) and/or recombinant murine WISP1 (500 ng/mL; R&D Systems, 1680-WS-050).

    Techniques: Expressing, Immunofluorescence, Western Blot

    Primary adult mouse cardiac fibroblasts were embedded in floating collagen gels and treated for 24 h with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (A) Representative gel images at 0 h and 24 h. (B) Quantification of gel area (% contraction; n = 4 isolations, 2 males & 2 females). (C–D) Confluent fibroblast monolayers were scratched and imaged every 2 hours for 24 hours using the same treatment conditions. (C) Representative bright-field images at time point 0 and 14. (D) Quantification of wound closure (% of gap closed relative to time 0; n = 8 isolations, 4 male & 4 female). Data are mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons; *p ≤ 0.05.

    Journal: bioRxiv

    Article Title: WISP1 drives a mechanically active immune modulatory and proliferative cardiac myofibroblast state

    doi: 10.64898/2026.02.17.706476

    Figure Lengend Snippet: Primary adult mouse cardiac fibroblasts were embedded in floating collagen gels and treated for 24 h with vehicle (Ctrl), WISP1 (500 ng/mL), TGFβ1 (10 ng/mL), or WISP1 + TGFβ1. (A) Representative gel images at 0 h and 24 h. (B) Quantification of gel area (% contraction; n = 4 isolations, 2 males & 2 females). (C–D) Confluent fibroblast monolayers were scratched and imaged every 2 hours for 24 hours using the same treatment conditions. (C) Representative bright-field images at time point 0 and 14. (D) Quantification of wound closure (% of gap closed relative to time 0; n = 8 isolations, 4 male & 4 female). Data are mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons; *p ≤ 0.05.

    Article Snippet: For treatments, CFs were seeded in 12- or 24-well plates in DMEM + 10% FBS and treated for 72 h with recombinant human TGFβ1 (10 ng/mL; R&D Systems, 7754-BH) and/or recombinant murine WISP1 (500 ng/mL; R&D Systems, 1680-WS-050).

    Techniques: